Steric exclusion and protein conformation determine the
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Solved 1. A protein was purified to homogeneity.
SOLVED: Size exclusion chromatography (gel filtration) is used to identify protein's molecular weight at 80,000 Dalton. This is the molecular weight of the native protein. SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the
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SOLVED: Explain how you would use Size exclusion chromatography to estimate the molecular weight of a protein molecule. Include an explanation of what information must be collected and how it is used.
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SEC-MALS for Absolute Molar Mass and Size Measurements
